DETERMINATION OF ANTIOXIDANT AND ANTIMICROBIAL BIOACTIVITY FROM PROTEIN HYDROLISATE OF COCOA POD HUSK (CPH)
Authors: Hannim Haffeza Haris and Mohamad Yusof Maskat
Journal Issue: Malaysian Cocoa Journal, Volume 14/2022
Keywords: Antioxidant, antimicrobial, bioactive protein, cocoa pod husk, protein extract
Published On: 10/05/2022
Abstract
In cocoa industry, the contents of cocoa beans have high demands, but the cocoa pod husks (CPH), waste of cocoa industry, were considered as are a material that not provide any economic returns and is regarded as an undesirable waste. As concern, this study will apply the cocoa pod husk to become as a high-value resource and will provide advantages in terms of nutrition and health for the consumers. This study was to investigate the bioactive protein of cocoa pod husk from clone of PBC 159 as a source for antioxidant and antimicrobial. Antioxidant activity was assayed by 3 different methods; DPPH, ABTS and FRAP. Antimicrobial screening and determination of MIC’s and MBC’s against tested microorganisms were undertaken using agar diffusion method and broth dilution methods, respectively. Results of antioxidative activities from 3 methods utilized in this study (DPPH, FRAP, ABTS) demonstrated clone PBC 159 potential for a natural antioxidant source. According to the results obtained, the protein extract of clone PBC159 showed significant DPPH activity with the IC50 value of 0.4557mg/ml, while IC50 of ascorbic acid as standard was 0.2865mg/ml. The protein extract of cocoa pod husk for PBC159 demonstrated the highest identified activity against Bacillus amyloliquefaciens. The MIC and MBC of the bacteria was 0.390 and 0.780 respectively. From this finding, shows the potential of cocoa pod husk protein in Antioxidant and Antimicrobial activity. In this finding also show the valuable contribution for obtaining cocoa pod husk (CPH), from an abundant, inexpensive, renewable, and sustainable source, to be apply as a high-value resource and will be provided advantages in terms of nutrition and health for the consumers.