Volume 14/2022 VALIDATION OF MULTIRESIDUE MYCOTOXIN ANALYSIS IN COCOA SEMI-FINISHED PRODUCTS Authors Badrul Hisyam, Z., Muhammad Isfaiz, I., Suzannah, S., Aznie Aida, A. and Muhd Fauzi, S. ABSTRACT efficient method based on acidified solvent extraction, dispersive solid phase extraction clean-up (d-SPE) and liquid chromatography quadrupole time of flight mass spectrometry (LC-QTOF-MS) analysis was validated for the analysis of ochratoxin A and aflatoxins (B1, B2, G1, G2) in cocoa butter and cocoa liquor. Extraction recoveries were ranged between 91.7 % to 113.8 % with RSDs below 12.3 % for cocoa liquor. On the other hand, cocoa butter gave extraction recoveries in the range of 89.0 – 108.2 % with RSDs below 9.5 %. The method limit of quantification was 2 ng/g for all mycotoxins with good linearity achieved with correlation coefficients better than 0.990, using matrix-matched standards to construct the calibration curve. The method was finally applied to cocoa butter and cocoa liquor samples obtained from the market, which were collected in 2019 – 2020. VIEW PDF DOWNLOAD