VALIDATION OF MULTIRESIDUE MYCOTOXIN ANALYSIS IN COCOA SEMI-FINISHED PRODUCTS
Authors: Badrul Hisyam, Z., Muhammad Isfaiz, I., Suzannah, S., Aznie Aida, A. and Muhd Fauzi, S.
Journal Issue: Malaysian Cocoa Journal, Volume 14/2022
Keywords: Mycotoxin, cocoa butter, cocoa liquor, LC-QTOF-MS, multiresidues
Published On: 10/05/2022
Abstract
efficient method based on acidified solvent extraction, dispersive solid phase extraction clean-up (d-SPE) and liquid chromatography quadrupole time of flight mass spectrometry (LC-QTOF-MS) analysis was validated for the analysis of ochratoxin A and aflatoxins (B1, B2, G1, G2) in cocoa butter and cocoa liquor. Extraction recoveries were ranged between 91.7 % to 113.8 % with RSDs below 12.3 % for cocoa liquor. On the other hand, cocoa butter gave extraction recoveries in the range of 89.0 – 108.2 % with RSDs below 9.5 %. The method limit of quantification was 2 ng/g for all mycotoxins with good linearity achieved with correlation coefficients better than 0.990, using matrix-matched standards to construct the calibration curve. The method was finally applied to cocoa butter and cocoa liquor samples obtained from the market, which were collected in 2019 – 2020.