METHOD VALIDATION OF COMPACT DRY ‘NISSUI’ TOTAL COUNT TECHNIQUE IN COCOA AND COCOA PRODUCTS
Authors: Hannim, H.H. and Norita, R.
Journal Issue: Malaysian Cocoa Journal, Volume 13(2)/2021
Keywords: Aerobic colony, Alternative method, Colony forming units, Rapid method kit, Validation
Published On: 1/11/2021
Abstract
In this study, method of Compact Dry Nissui for enumeration colony forming units of microorganisms was validating. The purpose of this method validation is to make sure the results obtained reliable throughout the study. This method is a rapid method kit for determining aerobic colony counts, which has been developed by Nissui Pharmaceutical Company for food application. The Compact Dry Nissui is a ready-to-use dry media sheet. The total aerobic colony count can be determined in the sample within 48 h of incubation. This method claim as the easiest and simple analysis tool for indicating colony forming units (cfu) for variety of cocoa and cocoa products. Therefore, the method was used to verify 4 different cocoa products sample; Chocolate Praline, Chocolate Block, Cocoa Powder and Cocoa Liquor. The verification parameters included in this study were Accuracy, Precision, Repeatability, Sensitivity/Specificity, and Limit of Detection (LOD). The results are as follows; Accuracy 98.8%, Precision 0.0918, Repeatability 9.18%, Sensitivity/specificity 100%, while for Limit of detection was 7.2/25g. From the result shown that this method fulfils the need of EURACHEM Microbiology Guideline®. Therefore, the Compact Dry Nissui plate can be considered as a convenient alternative method for routine microbiological testing in cocoa and cocoa products for enumeration and detection of aerobic microorganisms. The Compact Dry Nissui offers the advantage of the shorter time results compare to the conventional method.