MTT ASSAY EVALUATION OF THEOBROMA CACAO LEAF EXTRACT (COALEX-1) IMPACT ON BREAST CANCER CELL LINES ACROSS DIFFERENT SPECIES (4T1 AND MCF-7) IN VITRO
Authors: Zainal, B.1*, Muhammad Syameer Ezzat, F. A.2, Nur Shafira, M. S.3, Nur Farah, A. R.1 and Rasma Suzielawanis, I.1
Journal Issue: Malaysian Cocoa Journal, Volume 16/2024
Keywords: Theobroma cacao, breast cancer, extract, MCF-7, 4T1
Published On: 1/10/2024
Abstract
Breast cancer remains one of the leading causes of cancer-related mortality worldwide, with ongoing research focused on identifying novel therapeutic agents. In this study, the cytotoxic effects of Theobroma cacao leaf extract (CoaLeX-1) were evaluated on two distinct breast cancer cell lines: 4T1 (murine-derived) and MCF-7 (human-derived). The cytotoxicity was assessed using the MTT assay to determine cell viability after treatment with various concentrations of CoaLeX-1. A dose-dependent reduction in cell viability was observed in both cell lines, with more pronounced effects in MCF-7 cells. The half-maximal inhibitory concentration (IC50) was calculated to quantify the extract’s potency. These findings suggest that Theobroma cacao leaf extract may exhibit anticancer properties with potential species-specific efficacy. The potential of CoaLeX-1 as a natural agent for further investigation in breast cancer treatment is highlighted, particularly considering its differential impact on cell lines from different species.