PRODUCTION OF POWDERED SELECTED ENDOPHYTIC BACTERIA USING BIOREACTOR AND SPRAY DRYING TECHNIQUE
Authors: Ishak, Z. 1*, Ernie Eileen, R.R.2, Rahman, M.Z.A.3, Lea Johnsiul3, Norasekin Tamchek3, Roslina, M.S.3, Rosmawati, M.S.1, Nurfadzilah, M.4 and Mohd Anuar, S. 1
Journal Issue: Malaysian Cocoa Journal, Volume 16/2024
Keywords: Endophytes, bacteria, bioreactor, spray dry and powder product.
Published On: 1/10/2024
Abstract
Selected endophytic bacteria strains, including Bacillus subtilis, B. pumilus, Pantoea agglomerans, which produce plant growth hormones, were studied for optimal compatibility using bioreactor and spray dry techniques to establish the most effective powdered bacterial consortium as a biofertilizer in cocoa plants. The selected endophytic bacteria were grown separately in nutrient broth, exhibiting the greatest growth activity at the end of exponential phase, 24 hours into the cultivation period using a bioreactor (Techfors, Infors, Switzerland–30L). The bioreactor was run with 300C temperature, stirring speed of 150 rpm and aeration at 0.5 vvm respectively. The cultures were mixed into 10% (w/v) sterile skim milk as carrier and were spray-dried at constant inlet temperature of 120 ± 2oC using GEA MOBILE MINOR spray dryer. In conclusion, results obtained from this study has reveal that the selected endophytic bacteria possess the greatest production of powdered product using bioreactor, (Techfors, Infors, Switzerland–30L) fermenter and drying technique using GEA MOBILE MINOR spray dryer with specific parameters